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rcutsmarttm buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs rcutsmarttm buffer
    Rcutsmarttm Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 948 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rcutsmarttm+buffer/rCutSmart+Buffer/bio_rxiv__64898__2026__04__15__718726-75-4-6
    Average 99 stars, based on 948 article reviews
    rcutsmarttm buffer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Activity Assay:

    Article Title: A bacterial defense system targeting modified cytosine of phage genomic DNA.
    Article Snippet: All amplified products were verified by agarose gel electrophoresis and purified by the Universal DNA Purification Kit (TIANGEN). .. Nuclease activity assays Nuclease activity assays were conducted in a reaction volume of 10 μL containing 200 ng substrate DNA and 1 μM CMoRE proteins in rCutSmartTM Buffer (New England Biolabs). ..

    Article Title: A bacterial defense system targeting modified cytosine of phage genomic DNA.
    Article Snippet: .. Nuclease activity assays were conducted in a reaction volume of 10 μL containing 200 ng substrate DNA and 1 μM CMoRE proteins in rCutSmartTM Buffer (New England Biolabs). ..

    Polymerase Chain Reaction:

    Article Title: A novel RFLP-PCR method for the rapid diagnosis of Echinococcus multilocularis and different Echinococcus granulosus sensu lato species from tissue samples.
    Article Snippet: .. After confirmation of the presence a PCR product, 6 lL of the PCR product of each sample were incubated for 3 h at 37 C with 9 lL of a mixture containing AccI, DdeI and HinfI restriction enzymes (corresponding to 2.7 units for each of the three) and 1.3 lL of 10 rCutSmartTM Buffer (New England Biolabs, Ipswich, MA, USA) in 7.5 lL of nuclease-free water. .. The restriction fragments were separated by electrophoresis in 3% MetaPhor agarose gel (Lonza) for 45 min at 110 V for medium size (15 cm).

    Incubation:

    Article Title: A novel RFLP-PCR method for the rapid diagnosis of Echinococcus multilocularis and different Echinococcus granulosus sensu lato species from tissue samples.
    Article Snippet: .. After confirmation of the presence a PCR product, 6 lL of the PCR product of each sample were incubated for 3 h at 37 C with 9 lL of a mixture containing AccI, DdeI and HinfI restriction enzymes (corresponding to 2.7 units for each of the three) and 1.3 lL of 10 rCutSmartTM Buffer (New England Biolabs, Ipswich, MA, USA) in 7.5 lL of nuclease-free water. .. The restriction fragments were separated by electrophoresis in 3% MetaPhor agarose gel (Lonza) for 45 min at 110 V for medium size (15 cm).

    Article Title: Droplet-compatible single-cell DNA methylation sequencing with PreTIC
    Article Snippet: .. A master mix containing rCutSmartTM Buffer (NEB B6004) and Klenow Fragment (3’→5’ exo-) (NEB M0212M), was then added and the reaction (final 25 μL per well containing 0.5 mM dNTP, 8 ng/μL random hexamers, 25,000 cells, 1 U/μL Klenow Fragment (3’→5’ exo-), 1× rCutSmart buffer) was incubated at 4 °C for 15 minutes, and 37 °C (ramp 1 °C/s) overnight (16-20 hours) in a thermocycler. ..



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